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WebThis is the correct protocol if you are using the C2987H cells. If you are using the C2987I cells, please refer to this protocol. WebNEB 5-alpha Competent E.coli (High Efficiency) - 6x0.2 mlNew England BiolabsCatalog #C2987I SOC Outgrowth Medium - 100 mlNew England BiolabsCatalog #B9020S SOC Outgrowth Medium - 100 mlNew England BiolabsCatalog #B9020S 1 Thaw a tube of NEB 5-alpha Competent E. coli cells on ice until the last ice crystals disappear. pulve horsch porte