Stat1 chip

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August undefined, 2024

WebRabbit recombinant monoclonal STAT1 antibody [EPR23049-111] - ChIP Grade. Validated in WB, IP, ChIP, ICC/IF, Flow Cyt (Intra) and tested in Mouse, Human. Cited in 1 publication(s). Recombinant Anti-STAT1 antibody [EPR23049-111] - ChIP Grade (ab239360) Abcam WebChIP1: HeLa cells. General notes This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility …

IFN-γ selectively suppresses a subset of TLR4-activated genes …

WebApr 11, 2007 · Stat1 has been known as a regulator of gene expression and a mediator of IFNγ signaling in mammalian cells, while its effect in a heat shock response remains unclear. We used RNAi knockdown, point mutations, ChIP and promoter activity assays to study the effect of Stat1 on the heat-shock induction of the hsp90α gene under heat … WebJun 11, 2007 · Of the 34 loci known to contain STAT1 interferon-responsive binding sites, ChIP-seq found 24 (71%). ChIP-seq targets were enriched in sequences similar to known … We would like to show you a description here but the site won’t allow us. tops security customer service number

バイオビッグデータの「見える化」に成功、タンパク質の探索に …

WebSTAT1 Antibodies Antibodies that detect STAT1 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Immunoprecipitation. These antibodies target STAT1 in Human, Mouse, Rat, Canine and Non-human primate samples. WebMonoclonal Antibody for studying Stat1 (Tyr701) phosphate. Cited in 325 publications. Validated for WB, IP, IF, F, ChIP, ChIP. Available in 3 sizes. Highly specific and rigorously … WebSTAT3 belongs to the STAT family of transcription factors consisting of seven proteins, namely, STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b and STAT6. Activated STAT3 … tops seacon square

Phospho-STAT1 (Tyr701) Polyclonal Antibody - Thermo Fisher Scientific

Genome-wide profiles of STAT1 DNA association using …

WebNational Center for Biotechnology Information WebSTAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. tops seattle public schoolWebJan 5, 2011 · Basal levels of ∼25% (119/461) of these genes were found to be reduced by ≥3-fold in stat1 −/− MEFs compared to the levels for controls. Of these stringently selected genes, the expression patterns of 50 genes whose basal levels were most disrupted in the absence of STAT1 are shown in Fig. Fig.1B 1B (right panel). We defined these genes ... tops security limited

"WebJul 6, 2010 · While a large majority of genomic sites with high ChIP-seq signal is associated with a nucleotide sequence resembling a STAT1 binding site, only a very small subset of the over 5 million potential STAT1 binding sites in the human genome is … " - Stat1 chip

Stat1 chip

Diverse effects of Stat1 on the regulation of hsp90α gene under …

WebChIP Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. WebMar 4, 2016 · Columns depict STAT1 ChIP-seq data from mouse bone marrow–derived macrophages (BMM) that were either untreated or treated with IFNB or IFNG. Only RLTR30B elements that are bound by STAT1 upon IFNG treatment are shown. Bottom metaprofiles represent average normalized ChIP signal across bound elements.

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WebOct 4, 2024 · Chromatin immunoprecipitation (ChIP)-PCR and luciferase assays were used to detect the transcriptional activity of target genes of E2F2. Nuclear translocation of STAT1 and p65 were assayed by Western blot, co-immunoprecipitation (co-IP), and immunofluorescence experiments. WebMar 22, 2024 · THIF inhibited STAT1 phosphorylation and might bind to the STAT1 SH2 domain, leading to blockade of STAT1-STAT1 dimerization. ... and the anti-STAT1 antibody was used for ChIP-qPCR to measure the binding of STAT1 to the promoters. Data were analyzed and are plotted as percent (%) of input DNA. *p < 0.05 versus vehicle; **p < 0.01 …

WebChIP. Chromatin immunoprecipitations were performed with cross-linked chromatin from HT-1080 cells treated with IFN-γ (50 ng/ml) for 30 minutes and either Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time … WebThe Stat1 Antibody confirms silencing of Stat1 expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity. Show More. Western blot …

WebMar 9, 2024 · Validation of STAT1 and IRF1 ChIP-chip analyses. ChIP-chip was used to locate STAT1 and IRF1 sites at promoter proximal and distal sites of the genes of each … WebMonoclonal Antibody for studying Stat1 (Tyr701) phosphate. Cited in 836 publications. Validated for WB, WB, IP, IHC, IF, F, ChIP, ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Stat1 (Tyr701) (58D6) Rabbit Monoclonal Antibody (CST #9167) is ready to ship.

WebPhosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family …

WebSTAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, … tops scpWebStat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate ... Supershift and ChIP applications, sc-8394 X, 200 µg/0.1 ml. p-Stat1 (A-2) is available conjugated to agarose (sc-8394 AC), 500 µg/0.25 ml tops schools in ukWebNov 14, 2024 · ChIP-seqのビッグデータは、世界中から毎月約1,500件も報告されているが、これらのデータは毎月ChIP-Atlasに追加されているため、利用者は常に最新のデータを閲覧することが可能。 ... JUNなど、マクロファージ特異的遺伝子群にはSTAT1やSPI1などの転写因子が高 ... tops sewing ft myers flWebWe developed a method, ChIP-sequencing (ChIP-seq), combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing to identify mammalian … tops second nature notepadsWebJul 1, 2008 · Stat1 is activated in response to type I and type II interferons (IFNs) by phosphorylation at both Y701 and S727. On type I IFN (IFN-α and IFN-β) stimulation Stat1 … tops scandi knifeWebNov 26, 2024 · The C-terminal transactivation domain of STAT1 is required for an efficient recruitment of the components of the core Mediator complex to the IRF1 promoter, which harbors an open chromatin state under basal conditions, inducing transcriptional activation . Using ChIP assays, we found that IRF1 binds to the Mda5 promoter. tops senior dayWebWe compared the results of HATSEQ against two other popular chromatin immunoprecipitation sequencing (ChIP-Seq) methods and observed overlap in the detected ROIs but HATSEQ is more specific in delineating the peak boundaries. ... (STAT1) ChIP-Seq data-set, and show that the detected ROIs are highly specific for the expected STAT1 … tops sedgefield